| TI: |
Title
Cloning of a cDNA encoding a human DNA-binding protein similar
to ribosomal protein S1 |
| AU: |
Author
Eklund, EA; Lee, Seung-won; Skalnik, DG* |
| AF: |
Author Affiliation
Herman B Wells Cent. Pediatr. Res., Rm. 2600, Riley Hosp.
Children, 702 Barnhill Dr., Indianapolis, IN 46202-5225, USA
|
| SO: |
Source
Gene, vol. 155, no. 2, pp. 231-235, 1995 |
| IS: |
ISSN
0378-1119 |
| AB: |
Abstract
We report the cloning of a human complementary DNA that encodes
a protein which exhibits 36% identity and 62% similarity to
Escherichia coli ribosomal protein S1 (rpS1), including conservation
of four copies of an RNA-binding domain. This clone was obtained
by ligand-screening a lambda gt11 expression library with
a DNA probe derived from the CYBB gene promoter. Electrophoretic
mobility shift and Southwestern blot assays confirm DNA binding
activity of the protein, which exhibits preferential binding
to single-stranded and double-stranded DNA and a low binding
affinity for RNA. Hence, the rpS1 protein domain previously
identified as an RNA-binding motif can also serve as a DNA-binding
domain. |
| LA: |
Language
English |
| SL: |
Summary Language
English |
| PY: |
Publication Year
1995 |
| PT: |
Publication Type
Journal Article |
| DE: |
Descriptors
man; ribosomal protein S1; DNA-binding protein; cloning |
| CL: |
Classification
N 14414 Structure and sequence; G3 12780 Others |
| SF: |
Subfile
Biochemistry Abstracts 2: Nucleic Acids; Human Genome Abstracts
|
| AN: |
Accession Number
3755417 |
