Readers will find detailed perspectives on innovative cloning and sequencing strategies as well as gene regulatory sequences and proteins, RNA processing and transport, RNA editing DNA biosynthesis, transcription factors and oncoproteins. Other coverage includes RNA - biosynthesis, virus and phage infections, effect of hormones, role as a messenger, use of antisense methodology (oligonucleotides and RNA), and more DNA - effects of radiation, antibiotics and other agents, biosynthesis, virus and phage infections, cloning and sequencing, mutagenesis, and much more Enzymes - Ribozymes, RNases, DNases, polymerases, phosphorylases, DNA unwinding enzymes, methylguanine-DNA methyltransferase - and more.
Subject Coverage
Major areas of coverage include:
- Purines, Pyrimidines, and Analogs
- Nucleosides and Analogs
- Nucleotides, Nucleoside Di- and Triphosphates, and Analogs
- Oligonucleotides, Polynucleotides, and Analogs
- Transfer RNA
- rRNA and Ribosomal Proteins
- Protein Biosynthesis
- mRNA
- snRNA, snoRNA and ribonucleoproteins
- DNA
- Gene Regulation
- Gene Manipulation
- Enzymes
- Immunological Aspects
- Protein-Nucleic Acid Association
Dates of Coverage
Update Frequency
Monthly, with approximately 1000 new records added Size
Over 0 records as of May 2013 Print Equivalent
Nucleic Acids Abstracts (1989 - 2003) Supplier
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Ann Arbor, MI 48106-1346
Tel: +1-734-761-4700
Sample Record
| TI: |
Title
In vitro photooxidation of nucleic acids by ultraviolet A radiation
|
| AU: |
Author
Wamer, WG; Wei, Rong Rong |
| AF: |
Author Affiliation
Food and Drug Admin., HFS- 128, 200 C St., S.W., Washington, DC
20204, USA |
| SO: |
Source
PHOTOCHEM. PHOTOBIOL., vol. 65, no. 3, pp. 560-563, 1997 |
| IS: |
ISSN
0031-8655 |
| AB: |
Abstract
Although ultraviolet A radiation (UVA, 315-400 nm) has been shown
to induce oxidative stress in mammalian cells and skin, the critical
chromophore(s) and molecular target(s) involved have not been
identified. We examined the role of oxidative damage to nucleic
acids induced by UVA. To assess photooxidation of cellular DNA
and RNA by UVA, we irradiated human skin fibroblasts with up to
765 kJ/m super(2) UVA. Cellular DNA and RNA were isolated immediately
and enzymatically hydrolyzed to nucleosides. 8-Oxo-7,8- dihydro-2'-deoxyguanosine
(8-oxodG), a primary oxidation product in DNA, and 8-oxo-7,8-
dihydroguanosine (8-oxoG), resulting from hydroxylation of guanine
in RNA, were measured by HPLC with electrochemical detection.
We determined that irradiation of skin fibroblasts with levels
of UVA that produced moderate toxicity also resulted in significant
levels of guanine hydroxylation in RNA. Lower levels of photooxidation
were observed in DNA. These results suggest that measurement of
guanine hydroxylation in nucleic acids, particularly in cellular
RNA, may be a powerful tool for investigating the photobiological
activity of UVA. |
| LA: |
Language
English |
| SL: |
Summary Language
English |
| PY: |
Publication Year
1997 |
| PT: |
Publication Type
Journal Article |
| DE: |
Descriptors
U.V. radiation; DNA; RNA; 8-oxo- 7,8-dihydro-2'-deoxyguanosine;
8-oxo-7,8-dihydroguanosine; guanine; chromophores |
| CL: |
Classification
N 14630 Chemical reactions & interactions, including effects of
radiation |
| EM: |
Entry Month
9705 |
| SF: |
Subfile
Biochemistry Abstracts 2: Nucleic Acids |
| AN: |
Accession Number
4039552 |
Field Codes
The following field codes are found in the records of this database.
Here they are listed in alphabetical order by two-letter code. See Field Codes and Search Examples for detailed
descriptions and search examples.
| AB = Abstract |
IS = ISSN |
| AF = Author Affiliation |
LA = Language |
| AN = Accession Number |
NT = Notes |
| AU = Authors |
NU = Other Numbers |
| CA = Corporate Author |
OT = Original Title |
| CF = Conference |
PB = Publisher |
| CL = Classification |
PT = Publication Type |
| DE = Descriptors |
PY = Publication Year |
| ED = Editor |
SF = Subfile |
| EM = Entry Month |
SL = Summary Language |
| ER = Environmental Regime |
SO = Source |
| IB = ISBN |
SO = Source |
| ID = Identifiers |
TR = ASFA Input Center Number |
|
